Successful maintenance of suckling rat ileum in organ culture
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چکیده
منابع مشابه
Uptake and transepithelial transport of nerve growth factor in suckling rat ileum
Nerve growth factor (NGF) is necessary for the development of sympathetic and some sensory neurons. Milk may be a source of NGF for suckling young, but sites of intestinal absorption of the protein have not been identified. To determine whether NGF is transported across the absorptive epithelium of suckling rat ileum, we assessed binding, uptake, and transport of 125I-NGF by light microscopy an...
متن کاملIdentification of an endosomal antigen specific to absorptive cells of suckling rat ileum
A membrane fraction enriched in apical endosomal tubules was isolated from absorptive cells of suckling rat ileum and used as an immunogen to generate anti-endosome monoclonal antibodies. By immunofluorescence, one of these antibodies bound exclusively to the region of the apical endocytic complex in ileal absorptive cells, but not to other cell types. Immunoblot analysis showed the antigen as ...
متن کاملTransepithelial transport of epidermal growth factor by absorptive cells of suckling rat ileum.
Epidermal growth factor (EGF), an acid-stable peptide present in rodent and human milk, is absorbed and promotes intestinal growth when fed to suckling rats. To determine whether absorptive cells of suckling rat ileum conduct selective transepithelial transport of EGF, we followed uptake of 125I-EGF from ileal loops by autoradiography and biochemical methods. Specific binding sites for 125I-EGF...
متن کاملSympathetic reinnervation of the rat iris in organ culture.
Reinnervation of sympathetically denervated rat iris by superior cervical ganglion cells has been demonstrated to occur in vitro. Return of [(3)H]norepinephrine uptake by irises incubated in contact with ganglia was associated with the reappearance of nerve fibers containing catecholamines that were demonstrable by fluorescent histochemistry. The reinnervating neurons appeared to follow the sam...
متن کاملDevelopment of embryonic rat eyes in organ culture
Previous studies on the differentiation of whole embryonic eyes in culture have been carried out mainly in the chick (Strangeways & Fell, 1926; Dorris, 1938; Harrison & Berry, 1959) and in one case in the rat (Tansley, 1933). Early postnatal intact eyes of rats and mice were cultured by Lucas & Trowell (1958), while more recently intact and trypsinized early eye rudiments (9 and 10 days) of mou...
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ژورنال
عنوان ژورنال: Gastroenterology
سال: 1978
ISSN: 0016-5085
DOI: 10.1016/0016-5085(78)93362-0